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C-01-01-EX

Sheet-formed scaffold for cell culture

CardioDISC^TM

- Research Use Only -

Each organ and tissue function in coordination with undifferentiated and mature cells, extracellular matrix (ECM) and growth factor. Especially, ECM is crucial for the differentiation and function of surrounding cells. ECM molecules influence the intracellular signals, which regulate proliferation, differentiation, migration, and function, through ECM receptors on the cell surface.

To study the physiological function of adherent cells, various ECM substitutes (e.g. ECM coats and 3D-scaffolds) consisting essentially of biological or chemical compounds have been used. However, it is reported that these substitutes are quite different from physiological environment. Moreover, low biocompatibility and difficulty of handling prevent researchers using these substitutes as a scaffold of cell transplantation.

CardioDISC^TM is a sheet-formed scaffold for cell culture derived from BSE free Japanese bovine pericardium. Our decellularization method (Fig.1) leads to the elimination of bovine antigen, the maintenance of native ECM structure and non-residual cytotoxicity. Therefore, CardioDISC^TM achieves high cell-affinity and high biocompatibility (Fig.2, Fig.3 and Fig.4).

- CardioDISC^TM supports cell culture experiments using various cell types.

- CardioDISC^TM is also able to be applied to cell transplantation experiments.

      Fig.1

Fig.2 Collagen in non-treated tissues and CardioDISC^TM was visualized by Masson-trichrome staining, showing that CardioDISC^TM had the collagen structure as same as non-treated tissues.


Fig.3 HUVEC (human umbilical vein endothelial cell) and P53LMACO1 (mouse aorta smooth muscle cell) were cultivated on CardioDISC^TM for 14 days, and then the cell numbers of those were counted. Good adhesion and proliferation were observed in both cells.

Fig.4 CardioDISC^TM were excised after 2 weeks of subcutaneous implantation inrat back, and observed by hematoxilin-eosin staining. Infiltration of inflammatory cells into CardioDISC^TM was hardly observed, and the adhesion of surrounding tissues was in good states.

Image of CardioDISC

Specifications
Product name	CardioDISCTM
Product code	C-01-01-EX
Contents	CardioDISCTM Φ21 mm x 12 sheets
Uses	Scaffold for cell culture, organ model and cell transplantation
Term of validity	6 months (4℃ : Sterility and viability of vascular endothelial cells )

April 27, 2009

 

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