News
- 2009.08.03
- Ghrelin ELISAs - The Global Standard of Ghrelin Assay
97751 Active Ghrelin ELISA
97752 Desacyl-Ghrelin ELISA
The Global Standard of Ghrelin Assay
1. What is Ghrelin?
Ghrelin is a peptide identified as endogenous ligand of the growth hormone secretagogue receptor (GHS-R). It is predominantly produced in the stomach and plays a key role in energy control by stimulating food intake.
Ghrelin is a 28-amino acid peptide. Active Ghrelin is acylated at the Ser3 residue with an n-octanoic acid. In contrast, Desacyl-Ghrelinis non-acylated and had been recognized non-active form cause the acylation is thought to be necessary for its biological activities.
But recent findings suggest Desacyl-Ghrelin is also possesses some kinds of biological activities.
It is also reported that ghrelin administration reduces insulin secretion. This suggests that a research on ghrelin may lead to a development of new therapy for diabetes mellitus.
2. Characteristic
◆ Global Standard for Ghrelin Study
“Active Ghrelin ELISA” and “Desacyl Ghrelin ELISA” are only one kit designed along with world wide patents;
- United States Patent 7,385,026
- European Patent 1197496 (Effective States：AT,BE,CH,CY,DE,DK,ES,FI,FR,GB,GR,IE,IT,LU,MC,NL,PT,SE)
- Australian Patent 784035
- Korean Patent 10-0827973
- Japanese Patent 3471780
◆ Detect Active/Inactive Ghrelin individually
Each kit can measure “Active- (n-octanoyled)” or “Non-active (Desacyl-)” Ghrelin specifically.
Adaption of specific monoclonal antibody on solid phase against n-octanoyled or unmodified 3-Ser region of Ghrelin N-terminal realizes those specific Active-/ Desacyl- Ghrelin capture.
Use-Friendly
- Our Ghrelin ELISAs need only 3:30 incubation time.
- Compare to RIA, ELISA can be taken without hot labs.
Long stability
- Compare to other EIA, our Ghrelin ELISAs are stable at 2 to 8℃ without refrigeration.
- Compare to RIA, Ghrelin ELISAs are more stable (a year after manufacturing, and expiry is mentioned in box surface).
3. Procedure
4. Kit Performance
Click here to jump to technical data
Click here to jump to Pretreatment and stability data
5. Research Field of Ghrelin (Results Probl Cell Differ. 2008;46:89-115.)
- Insulin resistance/ sensitivity
- Orexigenic
- Growth hormone release and energy homeostasis in vertebrates
- And following clinical research
- Growth hormone deficiency
- Eating disorder
- Gastrointestinal disease
- Cardiovascular disease
- Osteoporosis
- Aging, etc.
6. Product Description and Manual
