Home » Products » Antibodies » 65-012 anti-HIV-1 Gag p15 antibody, rabbit serum
- 65-012
- anti-HIV-1 Gag p15 antibody, rabbit serum
| Format | 0.09% sodium azide added to the antiserum |
|---|---|
| Storage | 4℃ |
| Package | 250uL |
| Species | rabbit |
| Download (protocol) | protocol |
| Note | Full Description HIV-1 Gag p15 is processed by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is further digested into nuclocapsid protein p7 and into p6 and p1 of unknown function. This digestion is promoted by the binding of HIV-1 genome RNA and the two Zn finger motifs that exist in the p7 region. The produced nucleocapsid protein p7 regulates the RNA function by directly binding to HIV-1 genome RNA (1). The product is prepared by immunizing rabbit with recombinant p15 protein which was over-expressed in E. coli with a plasmid carrying the Gag p15 coding region of HIV-1 virus, and highly purified by several steps of chromatography (2). Using this antiserum in Western blotting, the band of 15 kD corresponding to HIV-1 p15 was observed in the extract of the AIDS virus infected cells (Fig. 1) Antigen HIV-1 Gag p-15 Species Reactivity HIV-1, Human immunodeficiency virus type 1 Host Species rabbit Antigen Species HIV-1, Human immunodeficiency virus type 1 Applications Western blotting, and etc. Type polyclonal |
