- YK060
- Insulin ELISA Kit
| Format | ELISA/EIA |
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| Storage | 2 - 8℃ |
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| Package | 96 wells |
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| Species | Human/Rabbit/Canine |
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| Sample types | Serum |
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| Sample size | 25μL |
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| Sensitivity | 0.137 ng/mL |
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| Range | 0.137 - 100 ng/mL |
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| Protocol | 1st Incubation 16-20 hrs, 2nd incubation 2 hr, 3rd incubation 1 hr, 4th incubation 30 min. |
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| Download (protocol) | protocol |
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| Download (MSDS) | msds |
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| Note | Detection Target name
Insulin
Target Synonyms
Insulin, Insulin A chain, Insulin B chain
Description
This kit is a stable and convenient assay system for measurement of insulin (human, rabbit and dog) in serum samples. The processing of proinsulin, which occurs within the B cell yields insulin and C-peptide. Insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of insulin in blood is very important, and also provides valuable information to evaluate the pancreatic B cell function. This kit for determination of insulin concentration (IR-insulin) in serum samples of human, rabbit and dog is based on a sandwich enzyme immunoassay by using combination of guinea pig anti human insulin antibody (coated on plate), recombinant insulin standard, biotinylated guinea pig anti human insulin antibody and HRP labeled streptoavidin (SA-HRP). Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of insulin is calculated.
This product is useful for Diabetes / Obesity study.
Related products
Antibodies
Y370 Insulin (Human)
Peptides
4088-s Insulin (Human)
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