- YK090
- Glucagon EIA Kit (Rat / Mouse / Human)
| Format | ELISA/EIA |
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| Storage | 2 - 8℃ |
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| Package | 96 wells |
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| Species | Human/Mouse/Rat |
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| Sample types | Plasma |
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| Sample size | 100μL (or 50μL) |
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| Sensitivity | 50 pg/mL |
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| Range | 50 - 10000 pg/mL |
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| Protocol | 1st Incubation 20-24 hrs (50μL : 1st 44-48 hrs) , 2nd incubation 1 hr, 3rd incubation 20 min |
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| Download (protocol) | protocol |
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| Download (MSDS) | msds |
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| Note | Detection Target name
Glucagon
Target Synonyms
Glucagon, Gcg
Description
According to many studies on glucagon immunoassay, it has been established that the antibody against the C-terminal fragment (19-29) of glucagon has specific binding with pancreatic glucagon, whereas the antibody against the N- terminal fragment (1-19) of glucagon has specific binding with both of pancreatic and intestinal glucagon (total glucagon). Once, 30K by Unger et. al had been widely used as an antibody specific for the C- terminal fragment of glucagon, but Nishino, Shima and Yanaihara et. al succeeded in producing pancreatic glucagon specific antibody using synthetic peptide with the Cterminal fragment (19-29) of glucagon as immunogen in 1981. This EIA kit has been developed by using polyclonal antibody against glucagons (19-29), synthetic pancreatic glucagon as glucagon standard and biotinylated pancreatic glucagon as labeled antigen for the measurement of rat, mouse or human glucagon in plasma.
This product is useful for Diabetes / Obesity study.
Technical Data of YK090 Glucagon EIA Kit.pdf
YK090 Glucagon assay performance between 100uL and 50uL specimens
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