- MA001
- Markit M-Bradykinin
| Format | ELISA/EIA |
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| Storage | 2 - 10℃ |
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| Package | 96wells |
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| Species | rabbit |
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| Sample types | Whole Blood/urine |
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| Sample size | WB:5 mL urine:500μL |
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| Sensitivity | 7.2 - 5000 pg/well |
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| Range | 7.2 - 5000 pg/well |
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| Protocol | 1st Incubation 1 hr, 2nd incubation over night, 3rd incubation 30 min |
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| Download (protocol) | protocol |
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| Note | Detection Target name
Bradykinin
Target Synonyms
Bradykinin, Kallidin I
Description
Kallidinogenase as a cardiovascular active substance is one of kininogenases which release kinins by specifically acting on kininogens. It has been widely used in the medical treatment as a cardiovascular drug such as an antihypertensive drug. It is generally acknowledged that the measurement of kinin-degrading enzymes (kininases) and kinin-releasing activity in the bulk substance or preparations is important in the process and quality control in the manufacture of kallidinogenase preparations. Kininases are determined by the bioassay method with bradykinin (BK) as a substrate using the rat uterus or guinea pig ileum. On the other hand, for the measurement of kinin-releasing activity, the bioassay method is used in which the formed kinin is determined using kininogen as a substrate. However, these bioassay methods are complicated in procedure and require a great deal of labor. Therefore, we developed the kit for the assay of BK, MARKIT-A Bradykinin, based on enzyme immunoassay (EIA). However, as this method uses test tubes and requires two centrifugation steps, an improvement of the assay method has been awaited. After intensive studies, we have now developed MARKIT-M Bradykinin, a new kit for the assay of BK, which uses microstrip wells. This product is useful for Hypertension study.
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