Home » Products » Reagents / Proteins » 01-515 Diphtheria toxin mutant CRM197
- 01-515
- Diphtheria toxin mutant CRM197
| Format | 2 mg/ ml in 20 mM Tris-HCl (pH 7.2) 150 mM NaCl |
|---|---|
| Storage | -70℃ |
| Package | 200ug |
| Download (protocol) | protocol |
| Note | Species origin Diphtheria toxin mutant CRM197 Molecule Name Diphtheria toxin mutant CRM197 Type (General) peptide/protein Full Description This Diphtheria toxin CRM197 was highly purified from growth media of Corynebacterium diphtheriae mutant CRM197. CRM197 like wild-typediphtheria toxin is a single polypeptide chain of 535 amino acids (58 kD) consisting of two subunits linked by disulfide bridges . Binding to the cell surface of the less stable of these two subunits allows the more stable part of the protein to penetrate the host cell. Wild-type toxin catalyzes the ADP-ribosylation of eucaryotic elongation factor-2 (eEF2) by using NAD, thus inactivating this protein. However, CRM197 has an alteration of 52nd Gly to Glu and it has no ADP ribosylation activity nor toxicity to cells. |
