Home » Products » Reagents / Proteins » 01-521 Cholera toxin A subunit from Vibrio Cholerae
- 01-521
- Cholera toxin A subunit from Vibrio Cholerae
| Format | 1.6 mg/ml in 20 mM Tris-HCl pH 8.2、0.2M NaCl 10% glycerol filter-sterilized |
|---|---|
| Storage | -70℃ |
| Package | 50ug |
| Download (protocol) | protocol |
| Note | Species origin Vibrio Cholerae Molecule Name Cholera toxin A subunit Type (General) peptide/protein Full Description The main enterotoxin, known as cholera toxin, interacts with G proteins and increases cyclic AMP in the intestinal lining to open ion channels. As ions flow into the intestinal lumen (lining), body fluids (mostly water) flows out of the body due to osmosis leading to massive diarrhea as the fluid is expelled from the body. Cholerae toxin is a complex consisting of one molecule of A subunit (27.2 kD) and 5 molecules of B subunit (11.6 kD). It adsorbs to GM1 ganglioside on the surface of target cells by the B subunit and penetrates into cells where A subunit is dissociated and processed into A1, which constitutively activates adenyl cyclase activity of alpha subunit of Gs (a kind of GTP-binding protein) by ADP ribosylation activity. This product was highly purified from purified cholera toxin produced by V. cholerae Inaba 569B strain by gel-filtration (in the presence of urea) and ion-exchange chromatography. |
