Home » Products » Reagents / Proteins » 05-007 HIV-1 Gag p15
- 05-007
- HIV-1 Gag p15
| Format | 50% glycerol 20 mM Tris-HCl (pH 7.5) 50 mM NaCl 10 mM mercaptoethanol |
|---|---|
| Storage | -20℃ |
| Package | 20ug |
| Download (protocol) | protocol |
| Note | Species origin HIV-1, Human immunodeficiency virus type 1 Molecule Name HIV-1 Reverse transcriptase Type (General) peptide/protein Full Description HIV-1 Gag p15 is processed by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is further digested into nuclocapsid protein p7 and into p6 and p1 of unknown function. This digestion is promoted by the binding of HIV-1 genome RNA and the two Zn finger motifs that exist in the p7 region. The produced nucleocapsid protein p7 regulates the RNA function by directly binding to HIV-1 genome RNA (1). The product is over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag p15 coding region of HIV-1 virus, subtype B (2), and highly purified by several steps of chromatography (3). Its molecular size is 15 kD, same as that of p15 purified from AIDS virus particles (Fig 1). |
